abstract |
The invention provides a method for constructing a Cyp17a1 Cre animal model based on CRISPR-Cas9. The Cyp17a1 gene is modified to obtain a Cre enzyme expression model mouse. In the existing model animal construction method, there are random insertion positions, low construction efficiency, Cre enzyme Insufficiencies such as instability of expression. In view of the above technical defects, the present invention provides a method for constructing a Cyp17a1 Cre animal model. Using CRISPR-Cas9 technology, according to the principle of homologous recombination, the expression sequence of Cre enzyme is inserted before the stop codon TAG of the Cyp17a1 gene, accurately and efficiently. A new Cyp17a1 Cre tool mouse was constructed and the stability and specificity of its Cre enzyme expression were verified. |