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publicationDate 2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112410329-A
titleOfInvention Primer combination, kit and its application in early screening of ovarian cancer
abstract A primer combination, test kit and application thereof in early screening of ovarian cancer, each primer in the primer combination contains a specific sequence, the specific sequence contains as SEQ ID NO: 1 to SEQ ID NO: 278 at least one of the sequence shown or its complement. Since specific sequences can target tumor driver genes, the amplification and enrichment of mutation characteristics can be achieved; the designed specific primers are simulated and screened by the primer interaction algorithm, which effectively reduces the missed detection and data inconsistency caused by primer interaction. The above-mentioned first-round amplification primers are completed in one tube of amplification reaction, which effectively saves time, reagent usage and sample usage.
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