| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9de52bba13e16028dffa8775e3f3f28 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-47 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 |
| filingDate |
2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4626a061dd700c2000ee4863d229062
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa802671ef05caf77b835ecd5f8bd0c0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_160556fb9d09e2a54ffadbfa38c712c4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3283cd811cb537ddccec23a451a6c562 |
| publicationDate |
2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112410278-A |
| titleOfInvention |
A kind of method for efficiently synthesizing moenomycin A in Streptomyces albicans |
| abstract |
The invention discloses a method for efficiently synthesizing moenomycin A in Streptomyces albicans; it is based on the gene optimization of the heterologous expression host Streptomyces. Its heterologous host method, moenomycin A efficiently inhibits the synthesis of Gram-positive bacterial cell wall by acting on the active site of the peptidoglycan glycosyltransferase domain of bacterial cell wall aPBP. The moenomycine biosynthesis gene cluster was assembled by large fragments and the promoter was optimized by means of genetic engineering, and the moenomycin A resistance gene MaPBP was introduced into the optimized strain II to further improve the moenomycine tolerance of strain II. Finally, the heterologous expression yield of moenomycin A in strain LX03 increased by 110% compared with the starting strain I. The present invention not only describes the genetic engineering transformation method of large fragment gene clusters, but also opens a precedent for optimizing the Streptomyces. albus host for resistance genes to improve the yield of target compounds. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113444724-A |
| priorityDate |
2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |