http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112409477-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 |
filingDate | 2019-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112409477-B |
titleOfInvention | IgM purification method |
abstract | The present invention relates to the technical field of antibodies, in particular, to a method for purifying IgM, comprising: a. providing a sample containing IgM; b. obtaining a protein precipitate through at least one protein precipitation treatment and at least one protein concentration treatment; c. The protein precipitate is redissolved, and the impurity protein is removed by the octanoic acid-ammonium sulfate method to obtain a crude extract comprising the IgM; d. processed by hydrophobic chromatography to collect the permeate; e. by hydroxyphosphate limestone chromatography, Collect the eluate; and f. Purify the IgG-capture affinity chromatography resin and collect the permeate. The purity of IgM in the composition purified by the above method of the present invention is more than 95%, and the product is stable, precipitation is not easy to occur after cryopreservation, and the antibody activity is good. |
priorityDate | 2019-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.