http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112402355-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_10138fe3f2794ada7228aad7a2f9a013 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2800-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2800-805 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K8-345 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61Q19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61Q19-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K8-99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K8-60 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61Q19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61Q19-08 |
| filingDate | 2020-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9e735757697e00571c8c0af4765b837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d53a8d636b9b50d6b98781a237519d32 |
| publicationDate | 2021-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112402355-A |
| titleOfInvention | A kind of preparation method of compound preparation containing stem cells and secretion thereof |
| abstract | The invention belongs to the field of biotechnology, and in particular relates to a preparation method for a preparation containing stem cells and secretion complexes thereof, comprising the following steps: culturing stem cells derived from human body: including umbilical cord, placenta, adipose and other tissues, extracting and culturing stem cells; When the confluence of the cultured cells reaches 60% to 90%, digestion and passage are carried out, and the complete medium containing 5% to 10% of exosome-removed fetal bovine serum is used for passage to P3 to P8 generation expansion culture; the culture supernatant, Cells were removed by centrifugation; the supernatant was then filtered with a filter membrane, and apoptotic bodies were removed by centrifugation, and then placed in a centrifuge tube at low temperature for ultracentrifugation for 60-120 min to collect the concentrate; lyophilization protective agent was added, and frozen in an ultra-low temperature freeze dryer Dried, freeze-dried and stored as powder. The method of group centrifugation and ultrafiltration centrifugation adopted in the present invention prepares the stem cell secretion complex preparation, which has higher purity, simple operation and easy large-scale production. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114457010-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115786258-A |
| priorityDate | 2020-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 384.