http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112391409-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f850f296badc29b96d1270883dca5a76 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f941acf32dba2b914e1d3a8e41a1beb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69d2dffb97760e5519d775302ab1384c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2aa012e355a938e71c845be0054a9e53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ad83ab33281adc88a5179ff7b0e6c6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03c564caf733a3bea7fcb2dc423656df |
publicationDate | 2021-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112391409-A |
titleOfInvention | A kind of efficient genetic transformation technology of Ziping |
abstract | The invention discloses a high-efficiency genetic transformation technology of Ziping, comprising the following steps: 1) induction of Ziping callus; 2) callus growth; 3) co-cultivation after Agrobacterium infection; 4). Callus differentiation Cultivation: ①Selective culture under nursing culture conditions; ②ZT differentiation culture; The invention fills in the technical gap of the exogenous protein expression system of Rhizoma chinensis, and has the following advantages: (1) high transformation efficiency, 100% successful callus infection positive rate, callus differentiation rate as high as 51.3%, and transgenic plant rooting rate as high as 100% 100%; 2. the method is simple and easy to operate, and the process cycle is short, only 11-12 weeks; 3. the obtained transgenic plants are genetically stable; 4. the method of the invention has high yield, low cost, high safety, wide application and great benefit. The invention has great promotion value. |
priorityDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.