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filingDate 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f941acf32dba2b914e1d3a8e41a1beb0
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publicationDate 2021-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112391409-A
titleOfInvention A kind of efficient genetic transformation technology of Ziping
abstract The invention discloses a high-efficiency genetic transformation technology of Ziping, comprising the following steps: 1) induction of Ziping callus; 2) callus growth; 3) co-cultivation after Agrobacterium infection; 4). Callus differentiation Cultivation: ①Selective culture under nursing culture conditions; ②ZT differentiation culture; The invention fills in the technical gap of the exogenous protein expression system of Rhizoma chinensis, and has the following advantages: (1) high transformation efficiency, 100% successful callus infection positive rate, callus differentiation rate as high as 51.3%, and transgenic plant rooting rate as high as 100% 100%; 2. the method is simple and easy to operate, and the process cycle is short, only 11-12 weeks; 3. the obtained transgenic plants are genetically stable; 4. the method of the invention has high yield, low cost, high safety, wide application and great benefit. The invention has great promotion value.
priorityDate 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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