http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112390862-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2720-12122 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112390862-B |
titleOfInvention | Protein for detecting bluetongue, coding gene and soluble preparation method thereof |
abstract | The invention discloses a protein for detecting bluetongue, a coding gene thereof and a soluble preparation method. The invention firstly discloses a protein for detecting bluetongue, and the amino acid sequence of the protein is shown as 2 nd to 219 th position of SEQ ID No. 1. The invention further discloses a coding gene of the protein and a soluble preparation method thereof. The invention starts from the three-dimensional space structure characteristic of VP7 protein, cuts and rearranges the amino acid sequence of VP7 protein to obtain new VP7-N protein for detecting bluetongue, realizes the high-efficiency soluble expression of VP7-N protein in a prokaryotic expression system by introducing the coding gene of VP7-N protein into the prokaryotic expression system, has the characteristics of high expression yield and good solubility, in addition, does not reduce immunogenicity due to prokaryotic expression, has good immunoreaction characteristic, and provides high-quality protein for detecting bluetongue. |
priorityDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 383.