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filingDate 2020-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112379088-B
titleOfInvention Detection test paper for detecting lead residue based on gold nanoflower technology
abstract The invention belongs to the field of food safety immunodetection, and relates to gold nanoflower rapid detection test paper for detecting heavy metal lead based on monoclonal antibody. The heavy metal lead resisting monoclonal antibody 9B7 has affinity constant Kaff Reach 9.56 multiplied by 10 9 L/mol; the 50% inhibitory concentration IC50 for Pb-iEDTA reached 153 ng/mL. The gold nanoflower rapid detection test paper for detecting lead ions provided by the invention has the advantages that the detection time is 10min, the detection threshold (eliminating a T line) reaches 100ng/mL, and the detection limit (vLOD) reaches 1.562 ng/mL. The nanoflower material is combined with the heavy metal lead-resistant monoclonal antibody for the first time to prepare the gold nanoflower test strip, so that the gold nanoflower test strip has higher sensitivity, can realize high-sensitivity rapid detection of lead residues in grains, and ensures effective monitoring, prevention and control of the heavy metal lead residues.
priorityDate 2020-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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