| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b82e0333873efe87e95ad142a28e638 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08J2367-04 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-061
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08J9-40 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08L67-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-076
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate |
2020-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0ae9b6a19ee7698a7c41aebf3365a23 |
| publicationDate |
2021-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112375732-A |
| titleOfInvention |
3D suspension culture method for in-vitro amplification of spermatogonial stem cells |
| abstract |
The invention provides a 3D suspension culture method for in vitro amplification of spermatogonial stem cells. The method comprises the following steps: inoculating spermatogonial stem cell suspension on RGD polypeptide modified porous polylactic acid microspheres in a rotary biological reaction container, adding an improved RPMI-1640 culture medium, carrying out three-dimensional static culture at 30-34 ℃, starting a rotary system after spermatogonial stem cells are adhered to the RGD polypeptide modified porous polylactic acid microspheres, and carrying out three-dimensional dynamic suspension culture at 30-34 ℃. By using the method, the mouse SSCs can be amplified in vitro in a large scale on the premise of not adding feeder cells, and good stem cell proliferation activity and undifferentiated activity are obtained. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114480262-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114480262-A |
| priorityDate |
2020-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |