http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112359094-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b6d5f25e113f0abe6abf99f5fe774fec |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 |
| filingDate | 2020-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b87308afe2a3ac6361c0b8b90918112f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0894e4e0bda879e965207deba11cdf8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fbe9f06761c771a32cead4655c60071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c89e2a822355ce8aa105c1cba30a8ac7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_418189edf5d61790d8381a8f7a38d817 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63055eec6d44695a6553fc55526747e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41f0c0bc9417698eff45b55af6c9c31e |
| publicationDate | 2021-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112359094-A |
| titleOfInvention | DNA/Fe3O4Nucleic acid detection method combining net structure with magnetic three-phase extraction method |
| abstract | The invention discloses a DNA/Fe 3 O 4 The nucleic acid detection method combining the net structure with the magnetic three-phase extraction method comprises the following steps: (1) preparation of Fe 3 O 4 Nanosheets; (2) in Fe 3 O 4 Modifying single-stranded DNA on a nano-chip to obtain Fe 3 O 4 /ssDNA; (3) preparing hyperbranched DNA structural sample, mixing the hyperbranched DNA structural sample with Fe 3 O 4 /ssDNA mixing reaction to obtain DNA/Fe 3 O 4 A network structure; (4) mixing DNA/Fe 3 O 4 The system with the network structure is used as a water phase and mixed with an organic phase, a magnetic rod with extraction liquid drops is immersed into the organic phase to extract DNA/Fe 3 O 4 Taking out the reticular structure, monitoring the liquid drop which has the catalytic color reaction by using an ultraviolet-visible spectrometer, and analyzing the ultraviolet-visible spectrum data to obtain the content of the target nucleic acid in the sample to be detected. The method greatly reduces the detection limit of nucleic acid, has high practicability, and has the detection limit of microRNA-122 as low as 0.147aM, detection limit for H-DNA as low as 0.34aM, linear range of 0.5aM to 1pM and 1aM to 1pM (r) respectively 2 >0.995), the single-drop microextraction technology is utilized, the detection efficiency is high, the operation is simple, and the cost is low. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113322307-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113640240-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113640240-B |
| priorityDate | 2020-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419512635 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID962 |
Total number of triples: 22.