http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112359036-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-05001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P41-006 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 |
| filingDate | 2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112359036-B |
| titleOfInvention | Nitrilase mutant with improved catalytic activity and reaction specificity and application thereof |
| abstract | The invention discloses a nitrilase mutant and application thereof in synthesizing pregabalin chiral intermediate (S) -3-cyano-5-methylhexanoic acid by hydrolyzing racemic isobutyl succinonitrile, belonging to the technical field of biological engineering. The nitrilase mutant BaNITmut/V82L with the amino acid sequence shown as SEQ ID NO.4 or the double mutant BaNITmut/V82L/C237S with the amino acid sequence shown as SEQ ID NO.6 can catalyze high-concentration IBSN to hydrolyze, and the amide by-products are remarkably reduced. The method improves the nitrilase activity and the reaction specificity through the directed evolution technology, greatly reduces the industrial production cost, and has good application prospect in the industrial production of the pregabalin chiral intermediate (S) -3-cyano-5-methylhexanoic acid. |
| priorityDate | 2020-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 278.