http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112342271-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d0451e593692e9996ee1672449fb2fe |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8827 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
| filingDate | 2020-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77d581cc526686c05a4864be58f7c069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f14de98ab7587895f22019c54ba8c6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_541dab6c87f440b65282c268ad8877ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62d5c268a29646b9806bdce9e763c693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c4c44f0ca0665f3f296ba321665669c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5f6bc635906b3ebdafe21e9e0d226a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ec7d849dde23d4d4f2651e62541fc6c |
| publicationDate | 2021-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112342271-A |
| titleOfInvention | Method for quantitatively detecting content of free DNA (deoxyribonucleic acid) by isotope dilution mass spectrometry |
| abstract | The invention belongs to the technical field of biology, and particularly relates to a method for quantitatively detecting the content of free DNA by an isotope dilution mass spectrometry. Firstly, a peptide nucleic acid probe is disclosed, wherein the sequence of a nucleic acid part in the peptide nucleic acid probe is shown as SEQ ID NO: 1, and the peptide partial sequence in the peptide nucleic acid probe is shown as SEQ ID NO: 2, respectively. Also discloses a method for quantitatively detecting the content of free DNA single strands by an isotope dilution mass spectrometry method, which comprises the following steps: forming free DNA-biotin-streptavidin magnetic bead complexes; adding a peptide nucleic acid probe to capture the free DNA-biotin-streptavidin magnetic bead complex; carrying out enzymolysis; redissolving; indirectly calculating the DNA content in the sample according to the peak area ratio; correcting the DNA concentration to obtain a final concentration value. The method for quantitatively detecting the content of the free DNA single strand by the isotope dilution mass spectrometry has the advantages of high accuracy, reliable result and the like. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113376286-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113376286-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113063835-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113063835-B |
| priorityDate | 2020-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.