| abstract |
The invention relates to a porcine pseudorabies attenuated virus strain prepared by CRISPR/Cas9 and its application. The present invention uses the CRISPR/Cas9 system to knock out the gene fragments of US7, US8, US9 and US2 of the porcine pseudorabies virus strain to obtain a porcine pseudorabies virus attenuated strain (Pseudorabies virus) HB-1. ‑1 While retaining the immunogenicity, the toxicity is greatly reduced, and the vaccine is safe and will not cause disease in piglets. At the same time, after immunization, the protection rate of piglets reaches 100%. The attenuated porcine pseudorabies strain HB-1 provided by the invention provides material basis and technical support for the clinical prevention and control of porcine pseudorabies and the elimination of porcine pseudorabies virus in pig herds, and has broad application prospects. |