http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112342156-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-552 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-099 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-10 |
filingDate | 2020-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112342156-B |
titleOfInvention | A kind of preparation method and application of Bordetella outer membrane vesicles |
abstract | The embodiment of the invention discloses a preparation method and application of Bordetella outer membrane vesicles, and belongs to the field of biotechnology. A preparation method of Bordetella outer membrane vesicles, comprising the following steps: 1) culturing Bordetella bacterial liquid; 2) centrifuging at 10,000 g, 20 min, and 4° C., taking supernatant, and filtering through a filter; 3) using molecular weight A 100kDa ultrafiltration tube was concentrated at 5000g, 20min, and 4°C. The obtained concentrated solution was ultracentrifuged at 100,000g at 4°C for 2h, the supernatant was discarded, the precipitate was resuspended in TE Buffer, and frozen at -80°C. The process of the invention can improve the output of OMV, and provides reference for subsequent industrialized large-scale batch production. The present invention proves that OMV and OMV combined with aluminum adjuvant can induce animal body specific immune protection response through in vivo immune test, can be used for the development of Bordetella vaccine, and has broad application prospect. |
priorityDate | 2020-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 267.