http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112326637-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-76 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 |
| filingDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_376b00daa020eb6f0ec9a3efb3c53963 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a616f0763ff34c4808bd0cffcce02a42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e1146bc69f5cc165c683e6ade3a20ca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb1cc021c3c3f24b9ffcbfc32e5bd9c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_008c8d4e4b5bbac92bc1d261b8eec491 |
| publicationDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112326637-A |
| titleOfInvention | A kind of chemiluminescence biosensor for detecting 5-hydroxymethylcytosine, its detection method and application |
| abstract | The invention provides a chemiluminescence biosensor for detecting 5-hydroxymethylcytosine, a detection method and application thereof, and belongs to the technical field of molecular detection. The chemiluminescent biosensor of 5-hydroxymethylcytosine includes T4 bacteriophage β-glucosyltransferase, uridine diphosphate glucose, sodium periodate, aldehyde group reaction probe ARP, terminal transferase, hemoglobin and luminol solution. The hydroxymethylcytosine-glycosylation, periodate oxidation, biotinylation-isothermal signal amplification strategy designed in the present invention does not need to change the reaction temperature, nor does it require a special labeled nucleic acid probe or a specific template for signal amplification , which can detect 5hmC of any sequence in genomic DNA and does not involve isotopic labels or specific antibodies, eliminating radioactive hazards and misinterpretation of genome-wide mapping data in antibody-based experiments. This enables genome-wide analysis of 5hmC in a limited number of biological and clinical samples. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113637752-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113637752-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112961911-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112961911-A |
| priorityDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.