http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112326613-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0a6dc9360a583c222f41b3948facd9f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 |
| filingDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_507120e59aa5c28c23e3235e6eb5ad83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae6e7d8e5e8e41c28051577fd5ad1261 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1edb0d4943929b12c697be75edafaf6 |
| publicationDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112326613-A |
| titleOfInvention | Method for detecting thrombin content |
| abstract | The invention discloses a method for detecting thrombin content, which comprises the following steps: (1) drawing a fluorescence standard curve of the thrombin; (2) mixing the sample solution to be tested and DNA containing aptamer in buffer solution, incubating and adding AgNO 3 And Cu (NO) 3 ) 2 Mixing, incubating and adding NaBH 4 Mixing evenly and incubating; adding graphene oxide dispersion liquid, uniformly mixing, incubating and determining a fluorescence value; (3) and calculating the content of the thrombin in the sample liquid to be detected according to the standard curve. According to the invention, thrombin is specifically combined with DNA containing an aptamer, then DNA-Cu/Ag NCs are synthesized, and finally graphene oxide is added, so that background fluorescence is eliminated, non-specific adsorption of the graphene oxide on thrombin is avoided, and the steps of modifying the graphene oxide are reduced. The invention has the linear range of 2-10nM and the lowest detection limit of 0.25nM, and has the advantages of low cost, simple operation, high sensitivity, good selectivity, low toxicity, good biocompatibility, no labeling, etc. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114990191-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114990191-B |
| priorityDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 129.