http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112322700-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2019-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112322700-B |
| titleOfInvention | Construction method, kit and application of short RNA fragment library |
| abstract | The invention relates to the field of gene sequencing, in particular to a construction method, kit and application of a short RNA fragment library. The construction method includes pre-phosphorylation and dephosphorylation of the short RNA fragment sample, so that the phosphate group at the 3' end of the short RNA fragment is modified into a hydroxyl group, and the hydroxyl group at the 5' end is modified into a phosphate group to obtain a first product; and then Connect the 3' adapter to obtain the first ligation product; then perform dephosphorylation treatment to obtain the second product with a phosphate group at the 5' end; then connect the 5' adapter to obtain the second ligation product; then carry out reverse transcription and amplify to obtain The short RNA fragment library. The kit includes T4 polynucleotide kinase and RNA 5' pyrophosphohydrolase. The kit includes T4 polynucleotide kinase and RNA 5' pyrophosphohydrolase. The construction method and the kit can be used to obtain nucleic acid information of various short RNA fragments, and can be applied in the field of life sciences. |
| priorityDate | 2019-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 96.