| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f7db9565eff81c4dba42c5633ccb4feb |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0617
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0625 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d29d59eb84a1d37071af207dce9a2ee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1818a9e5fa2ee741e85ea81fd3d55a6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6822961c5b7dfecb20d1b990fa60b3f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9755421b9d503187dcce024ed5e0fb4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e759cdf25e5dacfa3aa646d4beab979e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55b9412fa01e520d928761a76254cd36 |
| publicationDate |
2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-112322689-A |
| titleOfInvention |
Application of FRT cell strain in preparation of preparation or kit for screening histamine H2 receptor modulator |
| abstract |
The invention relates to the field of biomedicine, in particular to application of an FRT cell strain in preparation of a preparation or a kit for screening histamine H2 receptor modulators. FRT cells endogenously express H2 at the mRNA and protein levels. Green fluorescence was observed in FRT cell membrane and cytoplasm by inverted fluorescence microscopy, confirming successful construction of a model of cells co-expressing CFTR and YFP-H148Q/I152L. Fluorescence quenching kinetics experiments prove that the model has the characteristic of cAMP activating chloride ion channels and can be used for screening modulators; the fluorescence slope value and the H2 receptor regulator form a dose-dependent curve relationship, and can be used for screening the H2 receptor regulator. The Z' factor is measured to be 0.7, and the established cell model can be used for high-throughput screening and has good stability. |
| priorityDate |
2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |