Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e4a2e28048a1c7eca235dbde9ee40a70 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-24243 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-18422 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-863 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
filingDate |
2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9fb7d0da16aef573b0c1e33e9a1a303 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f25122f1e0d78cdc774c600d0daf1127 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf8edab8d4ea569d1b597b4ccd43f647 |
publicationDate |
2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-112322658-A |
titleOfInvention |
Recombinant goat pox virus co-expressing peste des petits ruminants virus H and F proteins |
abstract |
The invention provides a recombinant transfer vector for constructing a recombinant goat pox virus. The transfer carrier mainly comprises: the gene comprises a left arm and a right arm of a TK gene, two reverse p7.5K promoters, a peste des petits ruminants virus H gene and a Peste des petits ruminants virus F gene which are respectively regulated and controlled, a p11K promoter, an enhanced green fluorescent protein (eGFP) gene and two forward Loxp sequences. The transfer vector and the goat pox virus are subjected to homologous recombination in a goat testicle cell to obtain a recombinant goat pox virus expressing eGFP, and an eGFP gene is specifically knocked out under the action of Cre enzyme to obtain the recombinant goat pox virus of potential co-expression peste des petits ruminants virus H and F proteins. |
priorityDate |
2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |