http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112322500-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-80 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-12 |
| filingDate | 2020-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112322500-B |
| titleOfInvention | Aseptic treatment method of Heterocurvium akashiwo |
| abstract | The invention provides a method for aseptic processing of Heterocurvium akashiwo, comprising the following steps: (1) weighing ampicillin, adding sterile water to mix and dissolve to obtain ampicillin solution; using a filter membrane to filter the obtained ampicillin solution Filtration; (2) preparing the H. akashiwo culture fluid to be sterilized; (3) adding the filtered ampicillin solution to the H. akashiwo culture fluid to be sterilized, and then carrying out aseptic culture; (4 ) During the aseptic culture process, the culture medium of Heterocurvium akashiwo was expanded regularly, and ampicillin solution was added during the expansion. The invention adopts antibiotic treatment combined with plate colony counting method to obtain relatively sterile Heterocurvium akashiwo culture, and long-term ampicillin treatment can improve the cell viability of Heterocurvula akashiwo. From the perspective of economic saving and environmental protection, it is suitable to use low final concentration of ampicillin for aseptic treatment, so as to maintain the algae culture in a relatively sterile state. |
| priorityDate | 2020-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 16.