| abstract |
The invention provides a nanoparticle for promoting tumor coagulation and dual-responsive drug release of enzyme/ATP, and a preparation method and application thereof. The nanoparticle uses the cross-linked hyaluronic acid modified by methacrylic anhydride as the shell layer, and the drug-loaded nanoparticle formed by self-assembly after modifying polylysine with 3-fluoro-4-carboxyphenylboronic acid as the core layer, and in situ Generate CaCO 3 nanoparticles; after drug administration is targeted to tumor tissue, Ca 2+ is rapidly released in the acidic environment of the tumor, induces tumor blood vessel coagulation, blocks the sugar supply of cancer cells, neutralizes and decomposes residual lactic acid in the tumor, and eliminates lactic acid. The resistance of cancer cells to apoptosis due to lack of glucose, and then the nanocarriers that enter the cancer cells escape from endosomes/lysosomes to the cytoplasm under the proton sponge effect of PLL, and interact with adenosine triphosphate (ATP) to rapidly release drugs to achieve tumor growth. Coagulation, delactation and ATP-responsive drug release synergistically kill cancer cells rapidly, which is of great significance for tumor therapy. |