http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112301118-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112301118-B |
| titleOfInvention | A method and kit for simultaneously obtaining RNA abundance and active RNA polymerase sites across the whole transcriptome |
| abstract | The present invention provides a method and kit for simultaneously obtaining RNA abundance and active RNA polymerase sites in the whole transcriptome. The method is based on the in vitro transcription of unmodified nucleoside triphosphates, N 6 -allyl adenosine triphosphate (a 6 ATP), and N 4 -allyl cytidine triphosphate (a 4 CTP) into nascent nucleoside triphosphates by active RNA polymerase In RNA, chemical treatment induces base mutation in the modified base during reverse transcription into DNA, and then the mutation site is identified by nucleic acid sequencing to obtain N 6 -allyl adenine (a 6 A) and N 6 -allyl adenine (a 6 A) and N 4 -allylcytosine (a 4 C) site, which is where the active RNA polymerase is originally located. The unmutated part is the original RNA, and the abundance of the new RNA can be obtained. The invention realizes the specific labeling of a 6 A and a 4 C in isolated chromatin for the first time, which can be located by means of mutation sequencing, and can obtain two kinds of information of active polymerase site and RNA abundance by one-time sequencing . |
| priorityDate | 2020-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 190.