| abstract |
The invention relates to the field of clinical microorganism detection, and provides a kit and a method for rapidly detecting and identifying mycobacteria in a clinical sample. The kit comprises 2 universal primers for amplifying a variable region sequence of 16S rRNA of mycobacteria, 1 specific primer for shielding the variable region of 16S rRNA of bacteria of the genus Rogowski, 1 specific primer for shielding the variable region of 16SrRNA of bacteria of the genus Corynebacterium, 1 sequencing primer for determining a sequence of a forward section of an amplified specific fragment of the mycobacteria, avidin-labeled magnetic beads, and a sequencing result and a database for comparing and judging the species. The kit can be used for detecting and identifying the mycobacteria in clinical samples, and can be used for clinical diagnosis of suspected mycobacteria infection in various clinical samples. Not only wins precious rescue time for clinical diagnosis and treatment, but also has the advantages of low cost, convenient operation and strong specificity. |