http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112280797-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01039 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1085 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-03047 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-0304 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8243 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2020-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112280797-B |
| titleOfInvention | A combined carrier capable of increasing the content of coenzyme Q10 in tomato and its construction method and application |
| abstract | The invention relates to a method for constructing a combined vector capable of increasing the content of coenzyme Q 10 in tomato. The steps include: obtaining E8 promoter sequence, tobacco Coq2 gene coding sequence, DPS gene coding sequence, HMGR2 gene coding sequence, UbiC gene coding sequence and hsp terminator sequence; construct the level 0 vector of each of the above six sequences; construct the level 1 vector of each of the above four genes; construct the level 2 vector. The present invention also relates to a method for producing tomato with increased content of coenzyme Q 10. The combined vector obtained by the above construction method is transformed into Agrobacterium GV3101 by heat shock, and wild-type tomato is transformed; after obtaining independent resistant callus, further Tomato lines with high content of coenzyme Q 10 were obtained by differentiation and regeneration. After cultivation and screening, the yield of coenzyme Q 10 in the fruit of the T3 generation of the tomato strain HUCD of the present invention can reach more than 7 times that of the wild type tomato fruit (~0.151 mg/g), and can be stably inherited with no difference in phenotype; that is, the present invention Coenzyme Q 10 content in the tomato line HUCD was significantly increased. |
| priorityDate | 2020-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 208.