http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112280737-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0d3b48b04ce7622a53811e42751a413 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M23-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M27-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-38 |
| filingDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf88581d894f5369a282affe19313225 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1a62cfd02db41253d25f15a3c74abc6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_deb615395cf882fe1469e5132a1c11ec |
| publicationDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112280737-A |
| titleOfInvention | Method for in vitro induced amplification and cryopreservation of immune cells |
| abstract | The invention relates to the technical field of biology, and particularly discloses a method for in-vitro induced amplification and cryopreservation of immune cells, wherein a single nuclear cell is subjected to first-stage amplification culture by using an amplification culture medium special for the immune cell to obtain a primary amplified immune cell; carrying out second-stage induction and amplification culture on the preliminarily amplified immune cells by using an induction culture medium special for the immune cells to obtain induced immune cells; performing third-stage activation and amplification culture on the induced immune cells by using an immune cell special activation culture medium to obtain a large number of immune cells with activation functions; freezing immune cells by using a freezing medium special for the immune cells to obtain the frozen immune cells; placing the frozen immune cells in a constant-temperature water bath device at 40 ℃ for thawing, and mixing with frozen recovery liquid of the immune cells to obtain recovery cell mixed liquid; and centrifuging the recovered cell mixed solution and removing the supernatant to obtain recovered immune cells. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113999771-A |
| priorityDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 110.