http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112255396-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f90c1df3857ee72900e0015c75caecd9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-745 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2020-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fc33566fa224db566989855df9792ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c11bed0892e70162165f65208319c75b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_407d9e280ff798fe11c855203a9c87de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba848dbe44f91d29f30db9449e3df34c |
| publicationDate | 2021-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112255396-A |
| titleOfInvention | Single-molecule mechanical method for measuring small-molecule drug inhibition protein nucleic acid interaction |
| abstract | The invention relates to a monomolecular mechanical method for measuring micromolecular drug inhibition protein nucleic acid interaction. First, a single-molecule reactor based on a DNA hairpin structure was designed and constructed. The stem portion of the DNA hairpin contains motifs that interact with proteins, and the handle portion is labeled with digoxin and biotin by polymerase chain reaction. Next, a protein that interacts with the DNA is prepared. And fixing the DNA hairpin structure between the magnetic beads and the glass sheet, and performing a DNA melting experiment in the microfluidic reaction tank by a monomolecular mechanical method. Finally, the influence of the small molecule drug concentration on the melting reaction of the DNA combined with the protein is systematically tested, a drug dose-response curve is drawn, the half inhibitory concentration is calculated, and the drug effect of the small molecule drug on the protein nucleic acid interaction is evaluated. The invention belongs to the fields of biomacromolecule interaction and monomolecular pharmacology, and develops a novel method for directly measuring micromolecular drug arrestin nucleic acid interaction for molecular biology research, drug development and pharmacological evaluation. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114200145-A |
| priorityDate | 2020-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 195.