http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112239764-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-11071 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-02 |
| filingDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112239764-B |
| titleOfInvention | Screening, identification and application of a carotenoid-cleaving dioxygenase-encoding gene involved in honeysuckle flower color change |
| abstract | The invention relates to a carotenoid cleavage dioxygenase LjCCD4 closely related to the flower color of honeysuckle and its encoding gene and function. The invention belongs to the technical field of genetic engineering. The invention discloses the open reading frame sequence of the honeysuckle carotenoid cleavage dioxygenase LjCCD4 gene and the amino acid sequence encoded by the gene. The present invention obtains LjCCD4 gene by screening based on honeysuckle genome and transcriptome data, constructs pET32a-LjCCD4 prokaryotic expression vector, and uses β-carotene and lutein as substrates to identify the catalytic mechanism of LjCCD4 in vitro. LjCCD4 can catalyze β-carotene and lutein to generate 10'-Apo-β-carotene aldehyde, β-ionone and 3-hydroxy-10'-Apo-β-carotene aldehyde, 3-OH-10', respectively ‑Apo‑alpha‑carotene aldehyde, 3‑hydroxy‑beta‑ionone, 3‑hydroxy‑alpha‑ionone. The invention discloses the internal molecular mechanism of the degradation of carotenoid pigments in honeysuckle and the change of flower color, and lays a foundation for the regulation of flowering period and molecular breeding of honeysuckle. |
| priorityDate | 2019-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.