http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112226484-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_653c418e41edbb4e1ce8bc5e53e70cec |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a616f0763ff34c4808bd0cffcce02a42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_720510ba6dc4e8a1c7fe6616965f17d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c81b783b5fe9704b5388c81d2ebaf34e |
| publicationDate | 2021-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112226484-A |
| titleOfInvention | Fluorescent biosensor and detection method and application of β-glucotransferase |
| abstract | The invention discloses a β-glucose transferase fluorescent biosensor, a detection method and application, including detection probe, endonuclease, exonuclease, helicase, polymerase, primer DNA and SYBR dye; the detection probe The needle is a hairpin structure formed by single-stranded DNA, the detection probe has a recognition site that can be recognized and cleaved by an endonuclease, the recognition site contains 5-hydroxymethylcytosine, and both ends of the single-stranded DNA are Modified with phosphate ester; after the detection probe is recognized and cut by endonuclease, it can be further cut by exonuclease; the helicase can unwind the stem of the hairpin structure; the primer DNA and the After the single-stranded DNA is complementary, double-stranded DNA is formed under the action of polymerase; the SYBR dye can specifically bind to the double-stranded DNA. The fluorescent biosensor provided by the invention has high sensitivity to β-glucose transferase, good specificity and short detection time. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114062672-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113406160-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113406160-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113249446-A |
| priorityDate | 2020-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 137.