http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112210609-A

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filingDate 2020-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba38f1db9e6f549cf7ed1fdff1650b91
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publicationDate 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112210609-A
titleOfInvention Real-time fluorescent PCR primers, probes, kits and methods for identification of puffer fish and their application in grilled fish fillets
abstract The present invention provides real-time fluorescent PCR primers, probes, kits, and methods for identification of puffer fish, and applications in the identification of puffer fish components in grilled fish fillets. The primers used for identifying pufferfish in the present invention include the upstream primer sequence Seq ID No.1 and the downstream primer sequence Seq ID No. 2. Compared with the prior art, the kit and the identification method of the present invention can accurately and rapidly carry out specific amplification and display fluorescence for the puffer fish of the genus Oriental puffer and the pufferfish, and the detection time is short, which can be completed in 5-6 hours. , the detection throughput is high, and the identification of 96 samples can be completed at the same time. The operation is simple, and the results can be observed only after DNA extraction and real-time fluorescent PCR amplification.
priorityDate 2020-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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Total number of triples: 21.