http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112210017-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4747 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate | 2020-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112210017-B |
| titleOfInvention | Group of tar death executor fusion proteins and tar death activity detection method based on luciferase fragment complementation analysis technology |
| abstract | The invention provides a group of pyroptosis performer fusion proteins and a pyropsis activity detection method based on a luciferase fragment complementation analysis technology, belonging to the technical field of cell biology. Pyrophoric performer fusion proteins include hGlucn-GSDME fusion proteins and hGlucC-GSDME fusion proteins. A group of pyro-death activity report plasmids comprise eukaryotic expression vectors respectively containing coding sequences of the two fusion proteins. The fusion protein or the reporter plasmid is applied to detection of GSDME-mediated cell apoptosis activity, preparation of a reagent or a kit for detecting the GSDME-mediated cell apoptosis activity or screening of a GSDME-mediated apoptosis inducer or inhibitor. The invention applies the luciferase fragment complementation analysis technology to the detection of the cell apoptosis activity for the first time, detects the aggregation of the GSDME activated hydrolysate N-GSDME, and provides a new technical method for researching the cell apoptosis. |
| priorityDate | 2020-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 619.