abstract |
Compositions for genome editing and site-directed integration in plants are provided, comprising microprojectile particles coated, treated or applied with recombinant DNA constructs for delivery to mature embryo explants from dried seeds, wherein The DNA constructs comprise sequences encoding one or more genome editing repeats. Also provided are methods of genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, as well as plants, plant parts, and seeds comprising edited genomes or site-directed integration produced by the disclosed methods. |