http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112198251-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-045 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-8675 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-86 |
| filingDate | 2020-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112198251-B |
| titleOfInvention | Method for the determination of plasma or tissue concentrations of six bufastenolide components in bufa skin-containing medicinal preparations |
| abstract | The present invention relates to a method for detecting the concentration of six bufastenolide components in plasma or tissue in preparations containing bufa skin. The method comprises the following steps: (1) preparation of a stock solution: lipobufaxin, bufalin, cinobufogen, cinobufulin, celinobufagin and xenobufalin are respectively dissolved in (50 ±5)% acetonitrile aqueous solution to prepare standard stock solution; dissolving reserpine in (50 ± 5)% acetonitrile aqueous solution to prepare reserpine stock solution; (2) preparation of working solution; (3) Preparation of the sample solution to be tested; (4) subjecting the sample solution to be tested obtained in step (3) to liquid chromatography separation; subjecting the sample separated by liquid chromatography to mass spectrometry analysis. In the detection process of the method of the invention, the chromatographic peak shape is excellent, and the channel of each compound has only a unique target peak, which avoids other interferences such as solvent effects, and has the advantages of simple operation, rapid analysis, high specificity and high resolution. |
| priorityDate | 2020-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.