http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112195163-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C02F3-342 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y111-01013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0065 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C02F3-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 |
filingDate | 2020-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112195163-B |
titleOfInvention | A manganese ion-independent mutant of manganese peroxidase and its application |
abstract | The invention discloses a manganese ion-independent mutant of manganese peroxidase and its application. The present invention carries out site-directed mutation to the 70th amino acid residue Arg and the 166th amino acid residue Glu on the basis of the I1-MnP1 amino acid sequence, and through constructing the expression strain, inducing expression, denaturing and renaturing and purifying, the mutant MnP is obtained (R70V/E166A). The mutant of the present invention can effectively decolorize anthraquinone dyes in a neutral pH environment independent of Mn 2+ , and has an improved tolerance to H 2 O 2 concentration, which is not effective for water bodies caused by anthraquinone dyes. Pollution control is of great significance. |
priorityDate | 2020-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.