http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112176034-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2020-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112176034-B |
| titleOfInvention | A rapid screening method for anemone toxin-producing microorganisms |
| abstract | The invention relates to the field of marine biotechnology, and discloses a rapid screening method for anemone toxin-producing microorganisms in view of the technical gap of a rapid screening method for anemone toxin-producing microorganisms, comprising the following steps: (1) obtaining strain DNA; ( 2) Obtain DNA probe hybridization solution: put the digoxigenin-labeled DNA probe solution in boiling water and then put it in an ice bath, take the DNA probe solution and add it to the hybridization solution; (3) Hybridization: put the DNA membrane Place in DNA probe hybridization solution and incubate for hybridization; place the hybridized DNA membrane in a container containing 2 x SSC solution for washing; (4) develop color; (5) judge the result. The DNA probe-colony in situ hybridization method is used for the rapid screening of sea anemone toxin-producing microbial strains, and the strains that can produce sea anemone toxin can be accurately screened through specific amplified DNA probes and simple and orderly operation steps , this method is simple and efficient, with short screening period and high accuracy. |
| priorityDate | 2020-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.