Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6967bba8150e0b546153ebb0fbc0eec9 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-62 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate |
2019-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c9ede1951f885435795f6ec87d38bc1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a3fd41d05b347666c1892e3854d2ea9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_585205b5fd61e6bd9b02ad671c534a2a |
publicationDate |
2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-112175971-A |
titleOfInvention |
Codon-optimized KRD gene and GDH gene and application thereof |
abstract |
The invention belongs to the field of enzyme catalysis, and particularly discloses a codon-optimized KRD gene and GDH gene and a method for preparing (S) -4-chloro-3-hydroxybutanoate by using the same in biocatalysis. The recombinant escherichia coli provided by the invention can simultaneously express ketoreductase and glucose dehydrogenase and express in a periplasmic space of cells, the expressed ketoreductase and glucose dehydrogenase have high enzyme activity, the constructed recombinant escherichia coli is used for preparing (S) -4-chloro-3-hydroxy ethyl butyrate through whole-cell catalysis, coenzyme or coenzyme factor is not required to be added, the problem that the traditional microbiological method for preparing (S) -4-chloro-3-hydroxy ethyl butyrate through catalysis is high in cost is solved, the conversion rate is up to 99.9%, and the ee value is up to 100%. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114591991-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114107355-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113136377-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113136377-B |
priorityDate |
2019-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |