http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112175920-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-26005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate | 2020-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112175920-B |
| titleOfInvention | A kind of method for two-phase extraction and purification of nuclease P1 |
| abstract | The invention relates to the field of biology, and specifically discloses a method for purifying nuclease P1 by aqueous two-phase extraction. Extract and purify nuclease P1. The method of the invention can efficiently separate and purify the nuclease P1, and the enzyme recovery rate of the nuclease P1 reaches 87.6%; and the method has low cost and can be suitable for large-scale industrial production. In addition, the purity of the nuclease P1 obtained by the method of the present invention is greatly improved, the specific activity is 4.6 times higher than that of the crude enzyme, and the impurities are less, so it can be used in the food industry. At the same time, both PEG and disodium nucleotides of the two water phase components can be recycled, which greatly reduces the cost. |
| priorityDate | 2020-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.