http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112175905-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0646 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2020-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112175905-B |
| titleOfInvention | A method for efficient knockout of CD96 gene in NK cells |
| abstract | The invention discloses a method for efficiently knocking out the CD96 gene in NK cells. The method uses a CRISPR/Cas9 gene editing system to introduce a complex of sgRNA and Cas9 protein into amplified NK cells by electroporation transfection wherein: the sequence of the sgRNA is selected from any one of the sequences shown in SEQ ID NO.1-10. The method of the present invention can realize the knockout of CD96 gene in NK cells, not only the knockout efficiency is high, but also the obtained NK cells that do not express CD96 can relieve the immunosuppressive effect of CD155+ tumor cells on NK cells. Wild-type NK cells show stronger anti-tumor activity in both in vivo and in vitro experiments, significantly improving the recognition and killing activities of NK cells on tumor cells, and are expected to be developed into safe and effective anti-tumor biological agents. |
| priorityDate | 2020-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.