http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112143688-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6967bba8150e0b546153ebb0fbc0eec9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01184 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-9901 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2019-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a3fd41d05b347666c1892e3854d2ea9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_585205b5fd61e6bd9b02ad671c534a2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c9ede1951f885435795f6ec87d38bc1 |
| publicationDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112143688-A |
| titleOfInvention | Construction and application of recombinant escherichia coli |
| abstract | The invention belongs to the field of enzyme catalysis, and particularly discloses construction and application of recombinant escherichia coli for preparing (S) -4-chloro-3-hydroxybutanoate through biocatalysis. The recombinant escherichia coli provided by the invention can simultaneously express ketoreductase and glucose dehydrogenase and express in a periplasmic space of cells, the expressed ketoreductase and glucose dehydrogenase have high enzyme activity, the constructed recombinant escherichia coli is used for preparing (S) -4-chloro-3-hydroxy ethyl butyrate through whole-cell catalysis, coenzyme or coenzyme factor is not required to be added, the problem that the traditional microbiological method for preparing (S) -4-chloro-3-hydroxy ethyl butyrate through catalysis is high in cost is solved, the conversion rate is up to 99.9%, and the ee value is up to 100%. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112941124-A |
| priorityDate | 2019-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 357.