http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112126656-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-61 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
filingDate | 2019-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2023-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2023-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112126656-B |
titleOfInvention | Protein stability detection system based on uroporphyrinogen Ⅲ methyltransferase CysGA |
abstract | The present invention relates to a protein stability detection system based on uroporphyrinogen III methyltransferase CysG A , wherein, including the C-terminal domain CysG A of Escherichia coli siroheme CysG and its homologous protein as a reporter protein, and The plasmid used to express the reporter protein in the host bacterium, the reporter protein has a site in the middle of its sequence that can be inserted into the protein to be tested, and the structure of the reporter protein after the site is inserted into the protein to be tested is: N-terminal sequence of reporter protein-test protein-C-terminal sequence of reporter protein. In addition, a method for detecting protein stability using the detection system, and a protein mutant with improved stability screened by the detection system for protein stability or the detection method are provided. |
priorityDate | 2019-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 390.