http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112121206-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a3dd4e2ea4af5781c0702795ef3ee0e1 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L2202-21 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L2-0088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-50 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L101-54 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L2-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-50 |
filingDate | 2019-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8709701ee67d093eeba9e8dd2acf8693 |
publicationDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112121206-A |
titleOfInvention | A kind of virus inactivation method of human placenta extract |
abstract | The invention discloses a virus inactivation method for a human placenta extract, including the following steps: 1) using Trizol RNA extraction reagent to extract total RNA of bovine pancreas; 2) RT-PCR amplification of the total RNA of bovine pancreas 3) Cloning to prepare the expression bacteria of bovine ribonuclease A; 4) immobilization of bovine ribonuclease A; 5) enzymatic inactivation of virus in placenta extract. The method of the invention adopts genetic engineering technology, clones and expresses RNase efficiently, and immobilizes the RNase, so that the placenta extract is passed through the immobilized RNase, so that the nucleic acid of the virus particles in the placenta extract is specifically enzymatically hydrolyzed and lost. The method of the invention has the advantages of high efficiency, high selectivity, mild catalytic reaction conditions, no pollution, low cost, easy operation, the enzyme can be reused repeatedly, and the product is single. |
priorityDate | 2019-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.