http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112111452-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0694 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 |
| filingDate | 2020-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112111452-B |
| titleOfInvention | A method for isolating and purifying eukaryotic ribosomes for in vitro translation |
| abstract | The present invention provides a method for isolating and purifying eukaryotic cell 80S ribosomes for in vitro translation. The isolated ribosome has a complete structure and uniform particles, and important ribosome structures such as ribosomal subunits and mRNA channels can be observed. This method is applicable to in vitro cultured cell lines as well as primary bone marrow mononuclear cells. The translation activity of ribosomes isolated from the in vitro cultured cell line HL60 was slightly higher than that of ribosomes in mouse bone marrow cells. At the same time, the method detected that the ribosome translation activity of bone marrow cells of mice knocked out the SNORD113-114 gene cluster was significantly reduced, which was consistent with the experimental conclusions obtained by other methods. |
| priorityDate | 2020-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.