http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112111016-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2f8374206e5267f7247b6450885cbcfb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2020-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05ae7919e8eb8d005ed211945239b33b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a7648ca9bcb3f6604ce0934fd727420 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e27ecfa3da1931fc14445831bac6379 |
| publicationDate | 2020-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112111016-A |
| titleOfInvention | A kind of extraction and purification method of Gp96-polypeptide complex (CTL-OT) |
| abstract | The invention relates to the technical field of heat shock protein preparation, in particular to a method for extracting and purifying a Gp96-polypeptide complex, comprising the steps of: grinding human-derived isolated placental tissue or tumor tissue into a homogenate, and then the homogenate Centrifugal extraction is also performed, and the supernatant is sequentially extracted and purified by Gp96 monoclonal antibody-Sepharose 4B affinity chromatography and HiTrap-Q Sepharose ion-exchange chromatography. In the present invention, the human isolated placenta or tumor tissue is used as the raw material, and the Gp96-polypeptide complex is obtained by Gp96 monoclonal antibody-Sepharose 4B affinity chromatography and HiTrap-Q Sepharose ion exchange chromatography, which significantly improves the Gp96-polypeptide complex. Extraction and purification of the substance to obtain content and efficiency. |
| priorityDate | 2020-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.