| abstract |
Provided is a method for constructing an antigen-presenting cell line without an endogenous HLA gene background, comprising: using a CRISPR/Cas9 gene editing system to knock out the endogenous HLA-C gene in a C1R antigen-presenting cell with a single HLA type, Wherein the C1R antigen-presenting cells comprise HLA-A, HLA-B and HLA-C genes, wherein HLA-A is not expressed, and HLA-B is substantially not expressed; and the antigen presentation without endogenous HLA gene background obtained by the method cell line. |