| abstract |
The invention discloses a method for detecting chromium by combining copper-doped carbon dot mimic enzyme with a fluorescent probe. The invention adopts sodium copper (Na) tetraacetate 2 [Cu(EDTA)]) The method is a precursor, copper-doped carbon dots (Cu-CDs) are synthesized by a pyrolysis method, based on the catalytic activity of a mimic enzyme of the Cu-CDs, o-phenylenediamine is used as a substrate, under the action of the mimic enzyme of the Cu-CDs, hydrogen peroxide is used for catalytically oxidizing o-phenylenediamine (OPD) to generate 2, 3-diaminophenol oxazine (DAP) with fluorescence, the existence of chromium (III) inhibits the catalytic reaction, the DAP fluorescence is reduced, and chromium (VI) is reduced to chromium (III) by 2' -hydrazine-bis-3-ethylbenzthiazoline-6-sulfonic Acid (ABTS), so that a novel chromium detection method is established, and the detection limit is 20 mug/kg. The method is applied to detection and analysis of chromium in environment and food, and the result conforms to the standard method. The method has the characteristics of high sensitivity and specificity, and has the advantages of simple and quick operation, no need of large instruments and the like. |