http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112094931-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A90-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2020-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112094931-B |
| titleOfInvention | A set of primers for amplifying the housekeeping genes of Cronobacter MLST typing traceability and the method and application of next-generation sequencing library construction |
| abstract | The invention provides a set of primers for amplifying the housekeeping genes of Cronobacter MLST typing traceability, a second-generation sequencing library building method and application, and relates to the technical field of second-generation sequencing. The present invention simultaneously establishes amplification primer sets for 7 housekeeping genes specified in the industry standard "SNT 4525.5-2016 Molecular Typing MLST Method for Pathogenic Bacteria in Exported Foods Part 5: Cronobacter". Based on the above-mentioned primer set, the present invention establishes a second-generation sequencing library construction method for Cronobacter MLST typing traceability, and at the same time, one PCR can realize the amplification of 7 genes, which can be directly used for library construction, and the operation is simple. The invention can carry out the sequencing work more conveniently, accelerate the speed of MLST typing, and reduce the workload. |
| priorityDate | 2020-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID962 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419512635 |
Total number of triples: 21.