http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112094842-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112094842-B |
| titleOfInvention | Annular blocking probe, amplification blocking mutation system containing annular blocking probe and application of amplification blocking mutation system |
| abstract | The invention provides a circular blocking probe, an amplification blocking mutation system containing the circular blocking probe and application thereof, wherein the circular blocking probe is a first binding sequence, a circular sequence and a second binding sequence according to the 5 '-3' direction, and the first binding sequence and the second binding sequence are inverted repetitive sequences; the 3' end 1 base of the circular blocking probe is complementary with the wild type site of the template; the 3' end of the annular blocking probe is modified with a blocking group; the circular sequence of the circular blocking probe is complementary to the template. According to the invention, the annular blocking probe is added into the amplification inhibition mutation system, the annular blocking probe is preferentially combined on the wild-type template before PCR starts, the wild-type template is closed, and then the specific primer is adopted to carry out PCR amplification on the mutation template, so that the specificity of PCR amplification is obviously improved, the linear range is enlarged, and the accurate quantification of the low-frequency mutation sample is realized. |
| priorityDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.