http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112094806-A

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filingDate 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4475454884d4c03412cf11b54fd7c13e
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publicationDate 2020-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-112094806-A
titleOfInvention A method for increasing the growth rate of equine skeletal muscle satellite cells in adherent culture and a cell oxidative stress culture model
abstract The invention discloses a method for improving the growth rate of equine skeletal muscle satellite cells adherent culture. The method comprises adding a gelatin solution to a culture container, and placing it in a 37° C. and 5% CO 2 environment for 1-3 hours to obtain surface formation A culture vessel with a gelatin layer; the horse skeletal muscle satellite cells are transferred to the culture vessel with a gelatin layer formed on the surface, and a proliferation medium is added, and cultured at 37° C. and 5% CO 2 for 1-3 hours , to obtain adherent cultured horse skeletal muscle satellite cells. The method of the invention accelerates the adherence speed of skeletal muscle satellite cells, shortens their adherence time, and accelerates the acquisition of skeletal muscle satellite cells. The cell oxidation model of the present invention provides favorable conditions and research basis for the research institutes such as muscle growth and muscle oxidative damage, and provides favorable conditions and research basis for skeletal muscle satellite cells to be used for muscle damage repair, and has great scientific research and social value.
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Total number of triples: 25.