http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112080538-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-60 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 |
filingDate | 2020-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-112080538-B |
titleOfInvention | Method for preparing icariside II based on enzyme catalysis |
abstract | The invention discloses a method for preparing icariside II based on enzyme catalysis, and belongs to the technical field of biology. The method comprises the following steps: (1) constructing recombinant engineering bacteria for expressing the cellobiohydrolase with an amino acid sequence shown as SEQ ID NO.1, and obtaining the cellobiohydrolase through induction expression; (2) carrying out conversion reaction by using cellobiohydrolase as a catalyst and icariin as a substrate, and separating and purifying reaction liquid after the reaction is finished to prepare icariside II. The biological method for preparing icariside II provided by the invention has the characteristics of low cost, simple operation steps, mild reaction conditions and the like, the conversion rate reaches more than 98%, and a foundation is laid for large-scale industrial production of icariside II. |
priorityDate | 2020-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 368.