http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112080532-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7e1be3d0ac5dcafb00a0d337444d049 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07B2200-07 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C273-189 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C273-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C275-24 |
| filingDate | 2020-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_868836a44890c0e5200106efebf6202a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c839fa61a976dbb24fe0507484831b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b89d2927a4169850a3db45642910def7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d48dda9e57a0d91f3d79b515c186babd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de2a8a8b1468bafdbf45b34572cfaa36 |
| publicationDate | 2020-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112080532-A |
| titleOfInvention | A kind of method for producing D-p-hydroxyphenylglycine by double enzymatic method |
| abstract | The invention discloses a method for producing D-p-hydroxyphenylglycine by a double-enzyme method, comprising: using DL-p-hydroxybenzene hydantoin as a substrate, in a solvent, under the action of hydantoin, in an anaerobic environment at The reaction is completed under the conditions that pH is 9.5-11 and temperature is 40-50°C to obtain an intermediate reaction solution; the intermediate reaction solution is subjected to ultrafiltration to remove impurities to remove proteins and pigments in the intermediate reaction solution, and the ultrafiltration permeate is collected. N-carbamyl hydrolase is added to the ultrafiltration permeate, and in an oxygen-free environment, the reaction is completed under the condition that the pH is 7.0-8.5 and the temperature is 50-55° C. to obtain D-p-hydroxyphenylglycine. The invention can improve the enzyme conversion efficiency of the substrate, reduce the amount of the enzyme used in the production, thereby reducing the cost and improving the competitive advantage of the enzymatic production of D-p-hydroxyphenylglycine. |
| priorityDate | 2020-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.