http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112079912-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112079912-B |
| titleOfInvention | High-activity canine alpha interferon recombinant protein and preparation method and application thereof |
| abstract | The invention belongs to the technical field of biological genetic engineering, and particularly relates to a high-activity canine alpha interferon recombinant protein, and a preparation method and application thereof. The preparation method comprises the steps of carrying out codon optimization on CaIFN-alpha before carrying out amino acid site mutation on the CaIFN-alpha to canine alpha interferon mutant CaIFN-alpha-Mut, CaIFN-alpha-Mut and protein label synthesis, then synthesizing on a plasmid vector, carrying out double enzyme digestion, cloning to an expression vector to obtain a recombinant plasmid, then carrying out enzyme digestion linearization, introducing into an expression host bacterium to obtain recombinant yeast, and then carrying out induced expression and chromatographic purification to obtain a target protein, namely the canine alpha interferon recombinant protein. Solves the problems of low content of the canine alpha interferon, low biological activity, short half-life period, poor stability, difficult formation of disulfide bonds, complex production process, high purification preparation cost and the like in the prior art, and can greatly reduce the production cost in large-scale production. |
| priorityDate | 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 159.